Genome editing in strawberry fruits by SpCas9 and LbCas12a. (A) Diagram of the anthocyanin-assisted screening and Agrobacterium infiltration transient expression techniques; (B) schematic representation of the pCas9-FvMYB10 vector; (C) phenotype of Agrobacterium infiltration with FvMYB10 in strawberry fruits; (D) frequencies of SpCas9 at 12 endogenous genomic targets in strawberry fruits; (E) schematic representation of the pCas12a-FvMYB10 vector; (F) frequencies of LbCas12a at eight endogenous genomic targets in strawberry fruits; (G) average efficiency of LbCas12a-mediated genome editing in strawberry fruits. An untreated fruit sample served as control in (D) and (F). Frequencies (mean ± S.D.) were calculated from three biological replicates (n = 3).