Genome editing in cotton leaves, sugar beet leaves and tomato fruits by SpCas9. (A) Schematic representation of the pCas9-GhMYB114 vector; (B) phenotype resulting from Agrobacterium infiltration with GhMYB114 in cotton leaves; (C) frequencies of SpCas9 at ten endogenous genomic targets in cotton leaves; (D) average efficiency of SpCas9-mediated genome editing in cotton leaves; (E) schematic representation of the pCas9-BvMYB1 vector; (F) phenotype of Agrobacterium infiltration with BvMYB1 in sugar beet leaves; (G) frequencies of SpCas9-mediated genome editing at eight endogenous genomic targets in sugar beet leaves; (H) average efficiency of SpCas9-mediated genome editing in sugar beet leaves; (I) schematic representation of the pCas9-SlAN2L vector; (J) phenotype resulting from Agrobacterium infiltration with SlAN2-like in tomato fruits; (K) frequencies of SpCas9-mediated editing at nine endogenous genomic targets in tomato fruits; (L) average efficiency of SpCas9-mediated genome editing in tomato fruits. An untreated fruit or leaf sample served as control in (C), (G) and (K). Frequencies (mean ± S.D.) were calculated from three biological replicates (n = 3).