Genome editing in tomato fruits with addition of BeYDV. (A) Schematic representation of the pCas9-SlAN2LYDV vector; (B) diagram of geminivirus-based replicons used to enhance genome editing efficiency; (C) phenotypes resulting from Agrobacterium infiltration with SlAN2L and SlAN2L+BeYDV in tomato fruits; (D) frequencies of SpCas9 and SpCas9+BeYDV at nine endogenous genomic targets in tomato fruits. P values were obtained using two-tailed Student’s t-tests. ^*P < 0.05, ^**P < 0.01, n.s., P > 0.05. (E) Average efficiency of SpCas9 and SpCas9+BeYDV mediated genome editing in tomato fruits; (F) schematic representation of the pPE-SlAN2L-YDV vector; (G) frequencies of prime editing at 14 endogenous genomic targets in tomato fruits. An untreated fruit sample served as control in (D) and (G). Frequencies (mean ± S.D.) were calculated from three biological replicates (n = 3).