REVIEW

Single-cell protein-DNA interactomics and multiomics tools for deciphering genome regulation

¹ State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
² Peking University-Tsinghua University-National Institute of Biological Science Joint Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
³ Institute of Molecular Medicine, College of Future Technology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China

^* Corresponding authors (emails: xionghaiqing@ihcams.ac.cn (Haiqing Xiong); ahe@pku.edu.cn (Aibin He))

Received: 13 October 2022
Revised: 22 December 2022
Accepted: 9 January 2023

Abstract

The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies, providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome. Recent advances in single-cell multimodality epigenomic technologies facilitate directly interrogating the regulatory relationship between multi-layer molecular information in the same cell. In this review, we discuss recent progress in development of single-cell multimodality epigenomic technologies and applications in elucidating cellular diversifications in development and diseases, with a focus on protein-DNA interactomics and regulatory links between epigenome and transcriptome. Further, we provide perspective on the future direction of single-cell multiomics tool development as well as challenges facing ahead.