Mechanisms of acoustic manipulation include acoustic radiation force and acoustics streaming. (A) Basic design of acoustofluidic devices, the device using bulk acoustic wave (upside schematics) featured by acoustic wave propagating in the bulk of the liquid, while surface acoustic wave device (down side schematics) featured by the Rayleigh wave propagating in the surface of the substrate [22]. (B) The mechanism of acoustic radiation force is the scattering of acoustic wave on the particle’s surface, indicating that the particle is directly manipulated by the acoustic field [39]. (C) The generation of acoustic streaming derives from the compression of fluid within the acoustic field; particle manipulation is achieved by generated streaming [43]. (D) Schematics of the dominance of acoustic radiation force and acoustic streaming in particle manipulation, a same acoustofludic device generates both effects, normally smaller particles are manipulated by acoustic streaming effect (up), while larger particles are manipulated by acoustic radiation force [53]. (E) Practical analysis of in a same acoustic field, larger particles are dominant by acoustic radiation force (left) and smaller particles follows the pattern of acoustic streaming (right) [54].