Acoustic manipulation achieved by acoustic streaming. (A) Lateral vortices generated particle trapping and separation effect, large particles are trapped by the vortices, while small particles migrated to side of the channel by inertial flow effect [98]. (B) Vertical vortices generated by acoustic resonator achieve particle separation, scale bar: 100 μm [99]. (C) Acoustic hologram derives a patterned acoustic field through programmed surface design, the patterned acoustic field generates vortex to trap particles in the center [103]. (D) Digital acoustofluidics by SAW generated vortices, by programing the activation of IDTs in the array droplet on the surface could be moved [94]. (E) Digital acoustofluidics achieved by bluk acoustic wave for flexible droplet manipulation [95]. (F) Zebrafish imaging system integrated with acoustofludic manipulation, where acoustic streaming provides fish trapping and lateral rotation for surrounding imaging [26].