Acoustofluidics manipulation with coupled acoustic radiation force and streaming. (A) Acoustofludics holographic manipulation with patterned acoustic field generated pressure crests for acoustic radiation force patterning, meanwhile acoustic streaming achieve particle transportation to the pressure crests [130]. (B) Wave-pillar excitation resonance derived virtual pillar array for deterministic lateral displacement-based separation, smaller particles can pass the virtual pillar with straight streaming line, while large particles are moved to edge areas, scale bar: 20 μm [131]. (C) Droplet in acoustic field presents both acoustic streaming based vortex and acoustic radiation force derived concentration, resulting ring patterning of particles [53]. (D) Acoustofluidics centrifugation achieved by coupling of acoustic streaming derived vortex and acoustic radiation force derived concentration, the coupling of the two effects enables a complicated moving trajectory of particle and nanometer sized particle separation, scale bar: 500 μm [133].